缝隙连接蛋白基因43在胃癌腹膜转移中的作用

Role of connexin 43 gene in peritoneal metastasis of gastric cancer

目的探讨缝隙连接蛋白基因43（Cx43）与胃癌临床病理特征的关系及在胃癌腹膜转移中的作用。方法采用免疫组织化学染色法检测2000年1月至2008年12月第三军医大学西南医院收治的32例诊断为胃癌合并腹膜转移患者的胃癌、癌旁及腹膜转移组织中Cx43的表达，并分析其与临床病理因素的关系。采用Spearman等级相关分析，Fisher确切概率法、χ2检验对结果进行分析。结果Cx43主要定位于细胞膜和细胞质，Cx43在胃癌组织中的阳性表达率为34％（11／32），在癌旁组织中为100％（32／32），在腹膜转移组织中为94％（30／32），胃癌组织与癌旁组织Cx43的阳性表达率比较，差异有统计学意义（χ2=28．350，P〈0．01），胃癌组织与腹膜转移组织Cx43的阳性表达率比较，差异有统计学意义（，=21．989，P〈0．01）；胃癌组织Cx43表达与患者性别、年龄无关（r=-0．030，-0．169，P〉0．05），与肿瘤分化程度、组织学分型和淋巴结转移有关（r=0．750，0．642，-0．357，P〈0．05）。结论胃癌组织中Cx43表达下调，腹膜转移组织中Cx43表达明显上调，可能是Cx43在胃癌腹膜转移中发挥正性促进作用。

Objective To investigate the relationship between the expression of connexin 43 （Cx43） and clinicopathological characteristics of gastric cancer, and to study the role of Cx43 in peritoneal metastasis of gastric cancer. Methods Thirty-two patients who had gastric cancer and with peritoneal metastasis had been admitted to Southwest Hospital from January 2000 to December 2008. Gastric cancer tissues, adjacent tissues and metastatic peritoneal tissues were obtained postoperatively, and the expression of Cx43 was detected by immunohistochemistry. The relationship between Cx43 expression and clinicopathological characteristics of gastric cancer was analyzed. All data were analyzed via Spearman rank correlation coefficient, Fisher exact probability and ehi-square test. Results The expression of Cx43 was mainly detected in the cell membrane and cytoplasm. The positive expression rates of Cx43 in gastric cancer tissues, adjacent tissues and metastatic peritoneal tissues were 34% （11/32） , 100% （32/32） and 94% （30/32） , respectively. There were significant differences in the Cx43 expression between gastric cancer tissues and adjacent tissues （χ2 = 28. 350, P 〈 0.01 ） , and between gastric cancer tissues and metastatic peritoneal tissues （χ2 = 21. 989, P 〈 0.01 ）. The expression of Cx43 did not correlate with age and sex of patients （ r = - 0. 030, - 0. 169, P 〉 0.05 ）, but with tumor differentiation, histological type and lymph node metastasis （r = 0. 750, 0. 642, - 0. 357, P 〈 0.05）. Conclusions There is a decreased expression of Cx43 in gastric cancer tissues and a up-regnlated expression of Cx43 in metastatic peritoneal tissues. Cx43 may play a positive role in the peritoneal metastasis.