黄芪杂多糖调节AA小鼠红细胞免疫黏附功能的研究

Effects of Astragalus heteropolysaccharides on erythrocyte immune adherence function of mice with adjuvant-induced arthritis

建立小鼠佐剂性关节炎(AA)模型,以雷公藤多苷(TG)为阳性对照,研究黄芪杂多糖(AHPS)对AA小鼠红细胞免疫黏附功能的影响及其效应机制。采用常规组织切片检查膝关节的病理组织学变化,PEG-6000沉淀、P-S染色法测定血清中循环免疫复合物(CIC)含量,荧光免疫组织化学法测定关节滑膜组织中免疫复合物(IC)含量,流式细胞术测定红细胞CR1数量,并进行足爪容积测定和关节炎症评分。结果显示:AHPS可显著改善AA小鼠的原发性和继发性症状;AHPS(250、500和1000mg·kg-1)可增加AA小鼠红细胞CR1的数量(P<0.01或P<0.05),并降低AA小鼠血液中CIC的含量和抑制IC在AA小鼠膝关节滑膜中的沉积(P<0.01或P<0.05);AHPS的作用呈浓度依赖性。结果表明,提高AA小鼠红细胞CR1基因的表达可能是AHPS对AA小鼠治疗效应的机制之一。

Astragalus heteropolysaccharides （AHPS） is obtained from the dried roots of Astragalus membranaceus （Fisch.） Bunge vat. mongholious （Bunge） Hsiao. In the present study, we observed its effects on erythrocyte immune adherence function in mice with adjuvant-induced arthritis （AA）. The mice were treated intragastrically with AHPS of 1 000, 500, and 250 mg·kg^-1·d^-1 separately and treated with tripterygium glycosides （TG） of 60 mg·kg^-1·d^-1 as positive control. The number of complement receptor type 1 （CR1） on erythrocyte, the concentration of circulating immune complex （CIC） in serum and the amount of immune complex （IC） deposition in synovium of knee joint were determined by flow cytometry, polyethylene glycol （PEG-6000） precipitation and ponceau S （P-S） staining and fluorescent immunohistochemistry respectively. The pathological change of knee joint was evaluated by histological section. The results showed that both AHPS and TG improved significantly the primary and secondary local or systemic symptoms of the mice with AA and reduced the synovium hyperplasia, inflammatory cell infiltrate, pannus and cartilage demolish of knee joint, and AHPS of 1 000, 500, and 250 mg·kg^-1·d^-1 could significantly increase the number of CR1 on erythrocyte, improve the elimination of CIC in the peripheral blood and reduce the deposition of IC in joint synovium in a dose-dependent manner （P 〈 0.01 or P 〈 0.05）. The results indicate that one of the therapeutic effective mechanisms of AHPS on mice with AA could be to increase gene expression of CR1 of mice with AA.