肝硬化大鼠原位肝移植血流动力学改变

Hemodynamic changes in cirrhosis rats after orthotopic liver transplantation

目的研究肝硬化大鼠异体原位肝移植术后血流动力学改变。方法雄性健康SD大鼠50只,采用腹腔及皮下注射四氯化碳(CCl4),同时饮用乙醇溶液诱导10周,制备肝硬化大鼠模型;按KAMADA二袖套法(门静脉和肝下下腔静脉)对肝硬化大鼠原位肝移植;观察肝移植前后门静脉血流动力学的变化。结果制备肝硬化大鼠模型36只;进行肝移植手术26次,术后成活并进行门静脉血流动力学指标检测12只。经统计学方法检验,肝移植术后门静脉压较术前明显降低[(1.60±0.10)kPavs.(1.70±0.25)kPa,P<0.05];门静脉血流量明显增加[(7.55±1.02)mL/minvs.(6.15±0.88)mL/min,P<0.05];门静脉阻力明显降低[(11.84×10-2±2.51×10-2)kPa/(mL.min)vs.(16.29×10-2±2.40×10-2)kPa/(mL.min),P<0.05]。结论异体肝移植术可改善肝硬化大鼠血流动力学指标。

Objective To investigate the hemodynamic changes in cirrhosis rats after orthotopic liver transplantation. Methods The cirrhosis model was constructed in 50 healthy male SD rats by intraperitoneal and subcutaneous injection with carbon tetrachloride （CCl4）, and induced by drinking alcohol solution for 10 weeks. Orthotopic liver transplantation with two-cuff technique was adopted to these rats. The hemodynamic changes were observed. Results Cirrhosis model was constructed in 36 rats, 26 of which then received liver transplantation. Finally 12 cirrhosis rats survived, and hemodynamics of portal vein were observed. The statistical analysis revealed that the portal venous pressure decreased significantly after the operation compared with pretransplant [（1.60 ± 0.10）kPa vs. （1.70±0.25）kPa, P〈0.05]; the portal vascular resistance increased significantly [（7.55 ±1.02） mL/min vs. （6.15±0.88）mL/min, P（0.05]; however, the portal venous flow decreased obviously [（11.84 × 10^-2±2.51×10^-2）kPa/（mL-min） vs. （16.29×10^-2±2.40×10^-2）kPa/（mL·min）, P〈0.05]. Conclusion The hemodynamics of cirrhosis rats ameliorate after orthotopic liver transplantation.