白术对小鼠移植性肉瘤S180的抑瘤作用及对Bcl-2基因表达的影响

Baizhu suppresses S180 sarcoma growth and inhibits Bcl-2 expression in tumor-transfected mice

目的探讨白术对小鼠S180肉瘤的抑制作用及对肿瘤凋亡相关基因Bcl-2表达的影响。方法昆明系小鼠60只,皮下注射S180细胞,同时分别给予白术、环磷酰胺处理小鼠,其后2周内测量各组肿瘤生长大小;采用RT-PCR法检测凋亡抑制基因Bcl-2的表达。结果白术组的肿瘤重量低于模型组而大于环磷酰胺组(P均<0.05),但白术抑制肿瘤生长的作用强度未呈现量效关系,且其抑瘤率不及环磷酰胺(P<0.05);与对照组相比,白术能显著抑制肿瘤Bcl-2基因的表达水平(P<0.05)。结论白术具有抑制肿瘤生长的作用,可作为肿瘤治疗的辅助药物。

Objective To study Baizhu＇s inhibitory effect on S180 sarcoma growth and its effect on Bcl-2 expression in tumor-transfected mice. Methods S180 cells were subcutaneously injected into 60 healthy Kunming mice. Meanwhile the mice were dealt with MTX or Baizhu, separately; the weight of tumor was measured in the following two weeks; the expression of Bcl-2 was detected by RT-PCR. Results The tumor＇s weight in Baizhu group was lower than that in model group, but higher than in MTX group （P〈0.05）, but Baizhu＇s inhibition was not associated with its dose （P〉0.05）, and Baizhu＇s rate of tumor growth inhibition was lower than that of MTX. Compared with that in control group, Bcl-2 expression was lower in Baizhu group obviously. Conclusion Baizhu can inhibit tumor growth and serve as an adjuvant drug in tumor therapy.