假型逆转录病毒载体高效介导兔平滑肌细胞基因转染

High-efficiency gene transfer into rabbit smooth muscle cells by pseudotyped retroviral vector

目的构建假型逆转录病毒载体MuLV／VSV-G，并用于转染兔平滑肌细胞，为兔平滑肌细胞基因转染寻找一种高效的载体。方法构建含有报道基因lacZ的假型逆转录病毒载体MuLV／VSV—G，测定滴度，并转染兔平滑肌细胞，观察其转导效率。并与MuLV的转导效率进行比较。结果构建的MuLV／VSV-G载体，病毒滴度为6～7．8×10^6CFU，转染兔平滑肌细胞的效率是（92±12）％。而MuLV的转导效率为（24土5）％。结论成功构建了假型逆转录病毒载体MuLV／VSV-G载体，该载体作为一种高效的载体可用于兔平滑肌细胞基因转染。

Objective To construct pseudotyped retroviral vector MuLV/VSV-G and transfer it into rabbit smooth muscle cells （SMC） in order to provide a high-efficiency vector for SMC gene transfer. Methods We constructed pseudotyped retroviral vector MuLV/VSV-G containing the previously reported gene lacZ, determined the titer, and determined the efficiency of gene transfer into SMC mediated by pseudotyped retroviral vector MuLV/VSV-G. Finally the transfer efficiency was compared with that by MuLV. Results MuLV/VSV-G vector was constructed. The titer of the vector was 6- 7.8 ×10^6CFU, the transfer efficiency was （92 ±12）% by using MuLV/VSV-G vector and （24±5）% by MuLV vector. Conclusion Pseudotyped retroviral vector MuLV/VSV-G which was constructed successfully is a kind of high-efficiency gene transfer vector in smooth muscle cells.