HIV-1儿童感染者CD3^(dim)CD56^+细胞数量及其受体变化的研究

Study on the changes of CD^(3dim)CD56^+ cell and its receptors in HIV-1 infected children

目的:研究人类免疫缺陷病毒-1(H IV-1)儿童感染者体内CD3d imCD56+细胞数量及部分细胞受体的变化。方法:采集11例经确认的H IV-1儿童感染者和10例H IV-1抗体阴性的健康儿童抗凝全血,应用流式细胞术对CD3d imCD56+细胞的数量和细胞上部分受体的表达进行测定。结果:H IV-1儿童感染者CD3d imCD56+细胞的数量显著多于健康儿童(P<0.01);H IV-1儿童感染者与健康儿童比较,CD16无变化(P>0.05),NKp30、NKG2D、K IR2DL1和CD244的表达极显著升高(P<0.01),NKp44、NKp46、K IR3DL1和NTB-A的表达显著升高(P<0.05),NKp80的表达极显著降低(P<0.01)。结论:H IV-1儿童感染者CD3d imCD56+细胞的数量显著多于健康儿童,并且细胞上受体的表达发生剧烈变化。

Objective To study the changes of CD3^aimCD56^＋ cell and its receptors in HIV-1 infected children. Methods Blood samples from 11 HIV-1 infected children and 10 healthy children were collected in EDTA coated vacutainer. The number of CD3^aimCD56^＋ cells and the expression of its receptors were determined by flow cytometry. Results The number of CD3^aimCD56^＋ cell in HIV- 1 infected children was higher than that in healthy children. Compared with healthy children ,no change was found in the expression of CD16 receptor in HIV-1 infected children ,but the expression of NKp30, NKG2D ,KIR2DL1 and CD244 receptors was significantly increased（P 〈 0.01 ）. The expression of NKp44, NKp46, KIR3DL1 and NTB-A in HIV-1 infected children was also increased（P 〈 0.05 ）, while the expression NKp80 receptor was decreased obviously（P 〈 0.01 ）. Conclusion The number of CD3^aimCD56^＋ cell in HIV-1 infected children was higher than that in healthy children. The expression of CD3^aimCD56^＋ receptors changes dramatically in HIV-1 infected children.