纤维蛋白凝胶与大鼠嗅鞘细胞的生物相容性

Biocompatibility of rat olfactory ensheathing cells and fibrin glue

背景:纤维蛋白凝胶已被证实为优质的生物材料,其在神经组织工程中亦得到应用,既往研究表明纤维蛋白凝胶可以做为一些移植细胞的支架材料。目的:观察纤维蛋白凝胶与大鼠嗅鞘细胞的生物相容性。设计、时间与地点:体外细胞学对比观察,于2007-08/2008-02在南通大学神经生物学研究所完成。材料:将纤维蛋白单体与催化剂混合制成纤维蛋白凝胶;取SD大鼠嗅球,行嗅鞘细胞原代培养。方法:实验分为对照组(单纯培养嗅鞘细胞)和纤维蛋白凝胶组(嗅鞘细胞与纤维蛋白凝胶联合培养)。培养1周后冰冻切片,行免疫荧光抗体标记,显微镜下观察及检测。主要观察指标:嗅鞘细胞形态学,细胞计数、胞体面积及细胞周长。结果:嗅鞘细胞接种到纤维蛋白凝胶后,大多数嗅鞘细胞能在纤维蛋白凝胶内生长,悬浮在纤维蛋白凝胶之中,以下层为主,接种7d后细胞胞体呈梭形或三角形,多为双极或三极。纤维蛋白凝胶组的嗅鞘细胞数明显多于对照组(P<0.05),且胞体相对较大(P<0.05),两组细胞周长差异无显著性意义(P>0.05)。结论:纤维蛋白凝胶与嗅鞘细胞具有良好的生物相容性,嗅鞘细胞能够在纤维蛋白凝胶内存活和增殖。

BACKGROUND： Fibrin glue has been demonstrated to function as a kind of biomaterial with high quality. It has been used in nerve tissue engineering and proved to be a kind of scaffold for some cells. OBJECTIVE： To explore the biocompatibility of fibrin glue and olfactory ensheathing cells （OECs）. DESIGN, TIME AND SETTING： An in vitro control trial based on cytology was performed at the Institute of Neurobiology, Nantong University from August 2007 to February 2008. MATERIALS： Fibrin glue was made of fibrin and catalyst, and OECs derived from rats’ olfactory bulb were normally primary-cultured. METHODS： OECs were divided into control （OECs clone spheres were cultured alone） and in fibrin glue （OECs clone spheres were cultured and combined with fibrin glue） groups. After 1 week of culture, the proliferation of OECs were observed by convert microscope and detected by S-100 immunofluorescence histochemical staining. MAIN OUTCOME MEASURES： OECs morphology, cell count, the area of the cell bodies and the perimeter of the cell were determined. RESULTS： OECs could survive, migrate in fibrin glue, and float in the fibrin glue in the lower layer. After 7 days of incubation, cell body exhibited fusiform or triangle, predominantly bipolar or tripolar. The number of the S-100 positive cells was more, and cell bodies were larger in fibrin glue group than control group （P 〈 0.05）. However, there was no obvious difference between two groups in cell perimeter （P 〉 0.05）. CONCLUSION： Fibrin glue has good biocompatibility with OECs, and OECs can survive and migrate in fibrin glue.