期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

多巴胺D_4受体对肾脏近曲小管上皮细胞Na^+-K^+-ATP酶活性的影响 被引量：3

Effect of D_4 Dopamine Receptor on Na^+-K^+-ATPase Activity in Renal Proximal Tubule Cells
原文传递
导出
摘要 目的研究多巴胺D4受体对肾脏近曲小管(RPT)上皮细胞Na+-K+-ATP酶活性的影响及其机制。方法以WKY大鼠的RPT细胞株作为研究对象,测定多巴胺D4受体激动剂PD168077对RPT细胞Na+-K+-ATP酶活性的影响,硝酸还原酶法检测一氧化氮含量。结果PD168077能明显降低RPT细胞Na+-K+-ATP酶的活性,该作用呈浓度和时间依赖性。多巴胺D4受体拮抗剂L745870(10-6mol/L)可阻断PD168077对RPT细胞Na+-K+-ATP酶活性的抑制。与对照组比较,PD168077刺激RPT细胞15 min后,一氧化氮的合成含量明显升高;一氧化氮合成酶抑制剂可以阻断多巴胺D4受体对Na+-K+-ATP酶的抑制效应,提示一氧化氮途径可能参与了多巴胺D4受体对RPT细胞Na+-K+-ATP酶的抑制作用。结论多巴胺D4受体能够抑制WKY大鼠RPT细胞Na+-K+-ATP酶的活性,该作用可能与一氧化氮途径有关。 Objective To investigate the effect of D4 dopamine receptor on Na^＋-K^＋-ATPase activity in renal proximal tubule （RPT） cells and the underlying mechanism（s）. Methods Immortalize RPT cells was used in the study to investigate the effect of D4 dopamine receptor agonist PD168077 on Na^＋-K^＋-ATPase activity in RPT cells from Wistar-Kyoto （WKY）rats. The NO production were determined by nitrate reductase method. Results D4 dopamine receptor agonist PD168077 inhibited Na^＋-K^＋-ATPase activity in a concentration-and time-dependent manner in WKY RPT cells. The effect was blocked in the presence of D4 receptor antagonist L745870 （10^-6 mol/L） and NO synthase inhibitor L-NAME. D4 dopamine receptor agonist PD168077 enhanced NO production. Conclusions D4 dopamine receptor agonist PD168077 inhibits Na^＋-K^＋-ATPase activity in RPT cells which may be related to increases in NO.
作者 何多芬 杨剑 周林 曾春雨
机构地区 第三军医大学大坪医院(野战外科研究所)心血管内科心血管病研究室
出处 《中华高血压杂志》 CAS CSCD 北大核心 2009年第12期1095-1098,共4页 Chinese Journal of Hypertension
基金 973计划前期研究专项(No.2008CB517300) 国家自然基金资助项目(30672199) 重庆市杰出青年科学基金(CSTC 2009BA5044) 国家杰出青年科学基金(30925018)
关键词 多巴胺D4受体 NA^+-K^+-ATP酶 肾脏近曲小管上皮细胞 Dopamine D4 receptor Na^＋-K^＋-ATPase Renal proximal tubule cells
分类号 R363 [医药卫生—病理学]
  • 相关文献

参考文献15

  • 1Banday AA, Lokhandwala MF. Dopamine receptors and hypertension[J]. Curr Hypertens Rep, 2008,10 : 268-275.
  • 2刘琰,曾春雨,何多芬,石伟彬,杨剑,于长青,杨成明,周林.内皮素B受体对肾近曲小管上皮细胞Na^+-K^+-ATP酶活性的影响[J].中华高血压杂志,2007,15(10):823-826. 被引量：2
  • 3Iwamoto T, Kita S. Hypertension, Na^+/Ca^2+ exchanger, and Na+ K+ -ATPase[J]. Kidney Int, 2006,69 : 2148 -2154.
  • 4Zeng C, Asico LD, Yu C, et al. Renal D3 dopamine receptor Stimulation induces natriuresis by endothelin B receptor interactions[J].Kidney Int, 2008,74 : 750-759.
  • 5Zeng C, Armando I, Luo Y, et al. Dysregulation of dopamine-dependent mechanisms as a determinant of hypertension: studies in dopamine receptor knockout mice[J].Am J Physiol Heart Circ Physiol, 2008,294 : H551-569.
  • 6Ricci A, Marchal-Victorion S, Bronzetti E, et al. Dopamine D4 receptor expression in rat kidney: evidence for pre-and postjunctional localization [ J ]. J Histoehem Cytochem, 2002, 50 : 1091- 1096.
  • 7Shin Y, Kumar U, Patel Y, et al. Differential expression of D2- like dopamine receptors in the kidney of the spontaneously hypertensive rat[J].J Hypertens, 2003,21 : 199-207.
  • 8Schafer JA, Li L, Sun D. The collecting duct, dopamine and vasopressindependent hypertension[J]. Acta Physiol Scand, 2000, 168:239-244.
  • 9Saito O, Ando Y, Kusano E, et al. Functional characterization of basolateraI and luminal dopamine receptors in rabbit CCD[J]. Am J Physiol Renal Physiol, 2001,281 : F114-122.
  • 10Wang X, Villar VA, Armando I, et al. Dopamine, kidney, and hypertension:studies in dopamine receptor knockout mice[J]. Pediatr Nephrol, 2008,23 : 2131 -2146.

二级参考文献10

  • 1Garrido MR,Iarael A.Endothelin ETB receptor signaling in the median eminence and subfornical organ of the rat brain[].Neuropeptides.2004
  • 2Abassi ZA,Ellahham Samer,Winaver Joseph,et al.The In-trarenal Endothelin Systemand Hypertension[].News in Physiological Sciences.2001
  • 3Hoff man A,Abassi ZA,Brodsky S,et al.Mechanisms of big endothelin-1-induced diuresis and natriuresis:role of ETB re-ceptors[].Journal of Hypertension.2000
  • 4Pollock DM,Pollock JS.Evidence of endothelininvolvement in the response to high salt[].AmJ Physiol Renal Physiol.2001
  • 5Liang M,Knox FG.Nitric oxide activates PKCαand inhibits Na+-K+-ATPase in opossum kidney cells[].American Journal of Physiology.1999
  • 6Vassileva I,Mountain C,Pollock DM.Functional role of ETB receptors in the renal medulla[].Journal of Hypertension.2003
  • 7Zeng C,Wang Z,Yu P,et al.D3dopamine receptor directlyin-teracts with D1dopamine receptor in i mmortalized renal proxi-mal tubule cells[].Journal of Hypertension.2006
  • 8Magro F,Fraga S,Ribeiro T,et al.Intestinal Na+-K+-AT-Pase activity and molecular events downstream of interferon-gamma receptor sti mulation[].British Journal of Pharmacology.2004
  • 9Gomes P,Soares-Da-Silva P.D2-like receptor-mediated inhibition of Na+-K+-ATPase activity is dependent on the opening of K+channels[].American Journal of Physiology-Renal Physiology.2002
  • 10Gariepy CE,Ohuchi T,Williams SC,et al.Salt-sensitive hy-pertensionin endothelin-B receptor-deficient rats[].The Journal of Clinical Investigation.2000

共引文献1

同被引文献51

  • 1曾春雨,杨志伟,吴丽娟,Laureano D.Asico,Robin A.Felder,Pedro A.Jose.D_3多巴胺受体基因敲除小鼠高血压产生机制的探讨[J].中华心血管病杂志,2005,33(12):1132-1136. 被引量：10
  • 2于长青,曾春雨,杨志伟.多巴胺D_5受体在血压调控中的作用[J].中华高血压杂志,2007,15(2):98-100. 被引量：5
  • 3Van Tol HH, Bunzow JR, Guan HC, et al. Cloning of the gene for a human dopamine D4 receptor with high affinity for the antipsychotic clozapine[J]. Nature, 1991,350(6319) :610-614.
  • 4Chio CL, Drong RF, Riley DT, et al. D4 dopamine receptor mediated signaling events determined in transfected Chinese hamster ovary cells[J]. J Biol Chem, 1994,269(16):11 813-11 819.
  • 5Ariano MA, Wang J, Noblett KL, et al. Cellular distribution of the rat D4 dopamine receptor protein in the CNS using anti-receptor antisera[J]. Brain Res, 1997, 752(1/2) :26-34.
  • 6Mrzljak L, Bergson C, Pappy M, et al. Localization of dopamine D4 receptors in GABAergic neurons of the primate brain[J]. Nature, 1996,381 (6579) :245-248.
  • 7Lahti RA, Evans DL, Stratman NC, et al. Dopamine D4 versus Dz receptor selectivity of doparnine receptor antagonists: possible therapeutic implications[J]. Eur J Pharmacol, 1993,236 (3) : 483 486.
  • 8Hillman DW, Lin D, Burnside B. Evidence for D4 receptor regulation of retinomotor movement in isolated teleost cone inner-outer segments[J]. J Neurochem, 1995,64(3) : 1326-1335.
  • 9O'Malley KL, Harmon S, Tang L, etal. The rat dopamine D4 receptor: sequence, gene structure, and demonstration of expression in the cardiovascular system[J]. New Biol, 1992,4(2): 137-146.
  • 10Ricci A, Bronzetti E, Fedele F, et al. Pharmacological characterization and autoradiographic localization of a putative dopamine D4 receptor in the heart[J]. J Auton Pharmacol. , 1998,18(2) : 115-121.

引证文献3

二级引证文献7

中华高血压杂志
2009年 第12期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部