黄海西北部沉积物中细菌群落16S rDNA多样性解析

Diversity of bacterial community in the sediments of the Northern Yellow Sea

为揭示黄海西北部海域不同站位沉积物中细菌群落的多样性,采用16SrDNA文库技术,对黄海西北部辽东半岛和山东半岛近岸5个站位的沉积物中不同季节的细菌群落特征进行解析和评价.结果表明,沉积物中微生物种类丰富,主要分布于5～10个已知的门,另外还存在大量未被认知的序列;各站位沉积物中优势菌均为变形细菌门,占文库序列的46%～60%,其中γ-和δ-变形细菌纲为门中优势类群,但在各个站位中存在明显系统发育学分歧.微生物种群在不同地理区域和季节都存在明显差异,不同功能类群与其特殊生境密切相关.

To reveal the diversity and difference of marine bacteria in the sediment of the Northern Yellow Sea, the 16S rDNA clone library technique was used to analyze the characteristics of bacterial community at different seasons from the five stations of the Northern Yellow Sea. The 16S rDNA clone library analysis indicated that the sediments contained high diverse of bacteria and mainly distributed in five to ten known phyla, and most of sequences were affiliated to uncultured bacteria. Phylogenetic analysis showed that the Proteobacteria was the dominant group in the sediments, accounting for 46%60% of total sequences, and γ-Proteobacteria and δ-Proteobacteria were dominant classes. However, all proteobacterial classes presented phylogenetical divergence at the five stations. The bacterial community diversity differed due to the geographical location and season, and different function microbial groups were closely related to their special habitats.