当归对四氯化碳肝损伤保护作用的电镜观察

Electron Microscope Observation of Radix Angelicae Sinensis on Protection with the Liver Injury Caused by Carbon Tetrachloride in Mice

目的：探索当归对四氯化碳（CCl4）肝损伤的保护作用。方法：50只小鼠随机分成5组：对照组（Ⅰ）、模型组（Ⅱ）、当归低剂量组（Ⅲ）、中剂量组（Ⅳ）和高剂量组（Ⅴ）。Ⅲ-Ⅴ组分别按5,10,20 g·kg^-1剂量sc当归水提醇沉液,Ⅰ、Ⅱ组注射等容量的生理盐水,每天1次,连续6 d。末次给药后2 h,Ⅱ-Ⅴ组ip 0.1%CCl4四氯化碳石蜡油溶液（0.1 mL·10g^-1体重）,24 h后处死小鼠,电镜观察肝组织结构线粒体和内质网变化。结果：当归各组肝细胞内线粒体及内质网结构受损伤程度较模型组明显减轻。结论：当归水提醇沉液对CCl4致小鼠急性肝损伤具有一定的保护作用。

Objective：Study the protection of Radix Angelicae Sinensis on liver injury caused by the carbon tetrachloride.Methods： 50 mice were randomly divided into five groups,with were contrast group（Ⅰ）,model group（Ⅱ）,low dose of Radix Angelicae Sinensis group（Ⅲ）,moderate dose（Ⅳ） and high dose（V）.Group Ⅲ～Ⅴ were given water extracting-alcohol precipitating solution of Radix Angelicae Sinensis according to the dose of 5,10,20 g·kg^-1 by subcutaneous injection respectively,and other two groups were given normal saline at the same volume dose for 6 days.At two hours after the last administration,group Ⅱ～Ⅴwere given the 0.1% paraffin oil solution of carbon tetrachloride by intraperitoneal injection,all the mice were sacrificed after 24 hours and to observe the change of liver tissue（especially mitochondria and endoplasmic reticulum） with electron microscope.Results： Injury degree of mitochondria and endoplasmic reticulum of liver intracellular in Radix Angelicae Sinensis groups were obviously alleviativer than that of the model group.Conclusion： Water extracting-alcohol precipitating solution of Radix Angelicae Sinensis has the function of protecting the mice＇s acute liver injury caused by carbon tetrachloride.