清纤方抗实验性大鼠肝纤维化的作用研究

Research on Qingxianfang's Function on Anti-hepatic Fibrosis in Rats

目的:探讨清纤方抗肝纤维化的治疗机制。方法:利用CCl4制备肝纤维化Wistar大鼠模型,分为正常组、模型组、清纤方组、秋水仙碱组。治疗8周后,用HE和Masson染色观察肝纤维化的形成,免疫组化检测Ⅰ、Ⅲ胶原,RT-PCR检测肝组织中转化生长因子β1(TGF-β1)mRNA的表达。结果:Ⅰ、Ⅲ胶原和TGF-β1mRNA的表达在治疗组均明显减少。结论:清纤方能有效减轻肝纤维化大鼠的肝脏损伤及纤维化程度,其机制可能与抑制肝内TGF-1β的表达有关。

Objective：To explore the therapeutic mechanism of Qingxianfang for anti-hepatic fibrosis.Method： Hepatic fibrosis of Wistar rat was established by injecting CCl4 and divided them into groups marked ＂model＂,＂Qingxianfang＂ and ＂colchicines＂.Another group of normal rat was used as ／!control／.After eight weeks of treatment,an observation of hepatic fibrosis is carried on by using HE and Masson pigmentation,immunohistochemistry and detecting the expression of collagen type I,type Ⅲ.TGF-β1 mRNA expression in hepatic tissue was determined by RT-PCR.Result： The collagen type Ⅰ,type Ⅲ and the expression of TGF-β1 mRNA reduced significantly in treatment group.Conclusion： Qingxianfang is effective for easing the liver damage and hepatic fibrosis condition in rats.The mechanism may be relative to the restraining of TGF-β1 expression in the liver.