摘要
针对利用葡萄糖和木糖合成2,3-丁二醇的Klebsiella pneumoniaeXJ-Li菌,优化培养基组成与发酵条件,围绕五、六碳糖共代谢的特点,探讨简单可行的代谢调控方法.结果表明,60g/L葡萄糖和40g/L木糖为碳源,5.75g/L NH4H2PO4为氮源,pH值维持在5.5,培养温度38℃,2,3-丁二醇浓度可达19.24g/L.确定了pH值调控和外源添加维生素C的调控方式,通过调节发酵过程中pH值于5.5左右,使2,3-丁二醇的产量提高了16.4%;添加60mg/L维生素C调节培养基的氧化还原状态,可使2,3-丁二醇的产量提高44.3%,批式发酵48h,2,3-丁二醇终浓度可达33.47g/L.
The production conditions of 2,3-butanediol by Klebsiella pneumoniae XJ-Li from glucose and xylose were optimized, and the regulation methods for 2,3-butanediol fermentation were also determined based on the metabolic features of hexose and pentose. The results displayed that suitable culture conditions were defined as glucose of 60 g/L, xylose of 40 g/L, ammonium dihydrogen phosphate of 5.75 g/L and pH of 5.5, and the concentration of 2,3-butanediol reached 19.24 g/L under the conditions when the strain was cultured at 38 ℃. The yield of 2,3-butanediol was increased by 16.4% after maintaining the pH about 5.5 in the fermentation process. And 60 mg/L of vitamin C was added into medium to regulate the redox state of system, which made the yield of 2,3-butanediol improved by 44.3% of 2,3-butanediol fermentation. 33.47 g/L of 2,3-butanediol was obtained in batch fermentation for 48 h under optimum culture conditions.
出处
《过程工程学报》
CAS
CSCD
北大核心
2009年第6期1174-1177,共4页
The Chinese Journal of Process Engineering