趋化因子受体CCR2b基因G190A多态性与脑梗死关系的研究

Relationship between the chemokine receptor CCR2b gene G190A polymorphism and cerebral infarction of Han population in Chinese Fujian district

目的探讨趋化因子受体CCR2b基因G190A多态性与中国福建地区汉族人群脑梗死(cerebral infarction,CI)的关系。方法采用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction frag-ment length polymorphism,PCR-RFLP)和DNA序列测定法检测200例CI及200名健康对照者趋化因子受体CCR2b基因G190A多态性分布,对两组之间的基因型频率和等位基因频率进行比较。结果CI组趋化因子受体CCR2b基因G190A多态位点的基因型和等位基因频率分布与对照组比较差异有统计学意义(P<0.05)。脑梗死组GG、GA、AA基因型频率分别为83.5%、15.0%、1.5%;G、A等位基因频率分别为91.0%、9.0%。正常对照组GG、GA、AA基因型频率分别为73.0%、22.5%、4.5%;G、A等位基因频率分别为84.3%、15.7%。脑梗死组GG基因型和G等位基因频率高于对照组(P<0.05)。结论趋化因子受体CCR2b基因G190A多态性与脑梗死的发病具有相关性,G等位基因可能是中国福建地区汉族人群脑梗死发病的遗传易感基因。

Objective To investigate the relationship between chemokine receptor CCR2b gene G190A polymorphism and cerebral infarction（CI） of Han population in Chinese Fujian district.Methods The G190A polymorphism in ccr2b gene was detected by polymerase chain reaction-restriction fragment length polymorphism analysis（PCR-RFLP） and DNA sequencing in 200 patients with CI（CI group） and 200 normal controls（NC group）.Results There were significant differences in frequencies of allele and genotype of CCR2b G190A gene polymorphism between CI and control groups（P〈0.05）.In CI group,genotypic frequency of GG was 83.5%,GA was 15.0%,AA was 1.5%.The allele frequency of G was 91.0% and A was 9.0%.In NC group,genotypic frequency of GG was 73.0%,GA was 22.5%,AA was 4.5%.The allele frequency of G was 84.3% and A was 15.7%.The frequencies of CCR2b 190GG genetype and G allele in CI group were siginificantly higher than that in NC group（P〈0.05）.Conclusions There was association between CCR2b gene G190A polymorphism and cerebral infarction（CI） in Chinese Fujian district Han populations.The CCR2b G190 allele may be a genetic risk factor for cerebral infarction of Han populations in Chinese Fujian district.