大鼠侧脑室注射链脲酶素可导致与散发性阿尔茨海默病类似的脑葡萄糖代谢障碍及细胞内Aβ1-40,Aβ1-42沉积增多

Intracerebroventricular injection of streptozotocin caused similar changes of rat brain to sporadic Alzheimer's disease with energy deficiency and increased Aβ1-40,Aβ1-42 positive cells

目的探讨大鼠侧脑室注射链脲霉素模型与散发性阿尔茨海默病(sporadic Alzheimer's disease,SAD)的相关性。方法S-D大鼠分别于第1天和第3天双侧侧脑室注射链脲酶素(STZ)建立模型。21d后检测琥珀酸脱氢酶(succinate dehydrogenase,SDH)、细胞色素C氧化酶(cytochrome-oxydase,CCO)和Na+-K+-ATPase、Ca2+-Mg2+-ATPase活性;高效液相法检测ATP浓度;免疫组化法检测Aβ1-40、Aβ1-42阳性细胞在海马区表达。结果STZ组大鼠脑内SDH、CCO和Na+-K+-ATPase、Ca2+-Mg2+-ATPase活性及ATP浓度与NS组相比均有显著降低。STZ组大鼠海马区Aβ1-40、Aβ1-42阳性细胞较NS组显著增多。结论大鼠侧脑室注射链脲霉素可导致ATP减少及脑内Aβ1-40、Aβ1-42表达增多等与SAD相类似的病理变化。

Objective To testify whether intracerebroventricular（icv） injection of STZ can cause energy deficiency with increased Aβ1-40,Aβ1-42 positive cells in rat brain that similar to changes of sporadic Alzheimer＇s disease（SAD）.Methods S-D rats were divided into groups to receive icv injection of STZ or normal saline（NS） bilaterally at 1st and 3rd day.After 21 days of operation,activities of succinate dehydrogenase（SDH）,cytochrome-oxydase（CCO） and Na^＋-K^＋-ATPase,Ca^2＋-Mg^2＋-ATPase were measured.ATP levels of groups were detected by high performance liquid chromatography（HPLC） method.Immunohistochemical method was used to detect Aβ1-40 and Aβ1-42 positive cells in hippocampus of rat brain.Results Rats received icv injection of STZ bilaterally showed decreased ATP concentration and increased positive cells of Aβ1-40,Aβ1-42 in the brain.Conclusions Introcerebroventricular injection of STZ can cause similar changes to pathogenetic disorders of SAD.