摘要
目的研究昆布提取物(Okam)对博莱霉素(Bleomycin)所致大鼠肺间质纤维化的抑制作用。方法Wistar大鼠经气管内注入博莱霉素诱导肺纤维化,随后每日灌胃给予Okam 25、50 mg·kg^(-1)进行干预。通过免疫组化检测胶原Ⅲ、TNF—α和血管内皮生长因子(VEGF)蛋白在大鼠肺组织的表达。结果Okam 50mg·kg^(-1)可明显降低胶原Ⅲ、TNF-α和VEGF蛋白表达;Okam 25 mg·kg^(-1)可明显降低胶原Ⅲ、VEGF蛋白表达,但TNF-α表达与模型组比较无差异。结论昆布提取物灌胃给药能减轻博莱霉素诱导的大鼠肺纤维化的程度,有可能被开发成为治疗肺纤维化的海洋药物。
Objective To study the anti-proliferative effects of Okam extract on bleomycin induced pulmonary fibrosis in rats and its underlying mechanisms. Methods Pulmonary fibrosis was induced in Wistar rats by intratracheal instillation of bleomycin. Then test groups received daily Okam at doses of 25 and 50 mg · kg^-1 orally and control group received normal saline. The expressions of collagen-Ш.tumor necrosis factor （TNF-α） and vascular endothelial growth factor （VEGF） proteins were determined by immunohistochemistry. Results Pulmonary fibrosis of test groups was improved as compared with that of model group. Protein expressions of collagen-Ш,VEGF and TNF-α in 50mg · kg^-1group were markedly lower than those in model group. Protein expressions of collagen-Ill and VEGF in 25 mg · kg^-1 group were lower than those in model group, while protein expression of TNF-α showed no difference with that in model group. Conclusion Okam extract alleviates bleomycin induced pulmonary fibrosis in rats by inhibiting the expressions of collagen-W, TNF-α and VEGF.
出处
《中国海洋药物》
CAS
CSCD
2009年第6期11-13,共3页
Chinese Journal of Marine Drugs
基金
国家自然科学基金资助课题项目(No.30271607)
