马粪海胆生殖腺营养成分的含量测定

Determination of nutrient constituents in the gonad of Hemicentrotus pulcherrimus

目的通过对采自黄海海域的马粪海胆生殖腺的营养成分分析,为马粪海胆资源的开发利用提供理论依据。方法水分、灰分、蛋白质均采用国标测定;总糖采用苯酚-硫酸法测定;粗脂肪采用索式提取法测定;脂肪酸采用气相色谱-质谱联用技术测定;无机元素采用原子吸收法测定。结果马粪海胆生殖腺水分含量64.20%,灰分含量12.70%,粗脂肪含量2.34%,蛋白质含量12.25%,总糖含量5.59%。脂肪酸成分中花生四烯酸和EPA含量较高。无机元素中Ca、Mg、Fe等营养元素含量较高。结论马粪海胆生殖腺的营养成分含量丰富,具有较好的开发利用价值。

Objective To analyze the nutrient constituents of the gonad from Hemicentrotus pulcherrimus. The results could provide a theoretical basis pulcherrimus. Methods The contents of the water, ash and for pro the development of the H. tein were determined by the national standard methods, total sugar by phenol-sulfuric acid method, rude fat by soxhlet＇s method, fatty acids by gas chromatography-mass chromatography, and inorganic elements by atomic absorption spectroscopy method. Results The contents of water, ash, rude fat, protein and total sugar were 64.20%, 12.70%, 2.34%, 12.25% and 5.59%, respectively. The contents of arachidonic acid and EPA were higher in fatty acids. Inorganic elements, such as Ca, Mg, Fe were also in higher level. Conclusion The gonad of H. pulcherrimus was useful in the exploitation of nutrient food.