AG3340抑制大鼠C6胶质瘤细胞增殖的初步研究

Inhibitory effect of AG3340 in the proliferation of C6 rat glioma cells

目的寻找一种既能抑制胶质瘤新生血管的形成，又能杀灭胶质瘤细胞的细胞因子，为临床肿瘤药物的筛选提供理论依据。方法培养传代大鼠C6胶质瘤细胞株以每孔1×10^4个细胞铺96孔培养板，分组加入AG3340[金属蛋白酶（MMPs）非肽类抑制剂，终浓度分别为0ng／ml、50ng／ml、100ng／ml、150ng／ml]，培养48h后，弃去培养液，每孔加入DMSO（二甲亚砜）150μl，振荡后用酶标仪570nm测定吸光度值（A值）。结果（1）随着加入AG3340浓度增加，部分大鼠C6胶质瘤细胞便会出现变圆、离壁、浮起直至死亡的现象。（2）对照组OD（取相同倍数视野中心）值：0．1645±0．015；低浓度组OD值：0．1443±0．011；中浓度组OD值：0．1358±0．011；高浓度组OD值：0．1213±0．014。总体及各实验组间比较，均有统计学意义（总体F=46．67，P〈0．01；各组间P〈0．05）。结论AG3340对大鼠C6胶质瘤细胞增殖有明显的抑制作用，且与浓度呈一定相关性，随着浓度的增加其抑制作用逐渐增强。

Objective To find a cellular factor that can inhibit the formation of tumor angiogenesis, and kill tumor cells,which provide a theoretical basis for clinical oncology drug screen. Methods Delivered C6 rat glioma cells （purchased from Shanghai cells institute） into single-cell suspensions with Dropper as each hole had 1 × 10^4 cells. Resurface 96-well culture plates. Added AG3340 containing different concentrations, then discarded the medium,and then added each hole DMSO150μl. After oscillating, the magnitude of absorbance （A value）was measured with microplate reader （ Bio-Rad Model 550 USA）. Results （ 1 ） Compared with the control group, AG3340 could inhibit cell proliferation. It had a gradual better effect as the concentrations increased, showing a dose-dependent character. When exposed in a higher concentration of AG3340, some of C6 rat glioma cells appeared rounded, slide away from the wall and float up, even to the phenomenon of death. （2）Between the experimental group （50 ng/ml group OD value ：0. 1426 ± 0.022; 100ng/ml group OD value：0. 1390 ± 0. 007 ; 150ng/ ml group OD value：0. 1226 ±0.006） with the control group （OD value：0. 165 ±0. 101 ） ,as well as various experimental groups. There was statistically significant （P 〈 0.05） by the Student-Newman-Keul test. Conclusions Inhibitory effect of AG3340 in the proliferation of C6 rat glioma cells is obvious and dose-dependent.