谷氨酸受体基因多态性与精神分裂症的相关性研究

Association study of schizophrenia and glutamic acid receptor gene polymorphism

目的探讨谷氨酸受体基因（GRIK2）多态性与精神分裂症的相关性。方法运用聚合酶链反应扩增及单核苷酸多态性的分子生物学技术，收集符合精神分裂症诊断标准的98个先证者及其父母组成的核心家系，对其谷氨酸受体基因rs1338165，rs728024，rs1408768，rs1335042多态性分型，进行精神分裂症的谷氨酸受体基因多态性的关联分析和传递不平衡（TDT）检验。结果rs1335042等位基因与精神分裂症相关联（P=0．026261），其中等位基因A是保护因素（Z=-2．222），G为危险因素（Z=2．222）；rs1408768、rs728024、rs1338165与精神分裂症无关联。结论谷氨酸受体基因rs1335042与精神分裂症相关联。

Objective To detect the genetic association between schizophrenia and rs1338165,rs728024, rs1408768,rs1335042 polymorphism at nucleotide of the glutamic acid receptor gene. Methods Observed in a sample of 98 parent/offspring trios where the proband net the American classification and diagnostic criteria for mental disorders. The Forth Revised Edition, criteria for schizophrenia using transmission/disequilibrium test （TDT） analysis. The polymorphism of glutamic acid receptor gene was detected with PCR methods and SNP typing in all nucleus families. Results The rs1335042 allele is connected with schizophrenia （P= 0. 026261 ）. Allele A was protective factor （ Z = -2. 222） and allele G was hazard factor （ Z = 2. 222 ）. The rs1408768, rs728024 and rs1338165 allele was independence with schizophrenia. Conclusion It shows an association between schizophrenia and the rs1335042 polymorphism at nucleotide of glutamic acid receptor gene in Chinese.