布氏田鼠封闭群建立及繁殖特性研究

Establishment of cleaning grade closed colony of Microtus brandti and reproductive traits analysis

目的:本研究通过药物净化与随机交配在实验室内建立清洁级布氏田鼠封闭群。方法:采用最大限度避免近交系统同时结合随机交配系统等方法对布氏田鼠进行了封闭群培育,巴氏染色法观察阴道涂片测定动情周期。结果:连续繁殖6代得到清洁级布氏田鼠封闭群。布氏田鼠的发情周期为5天,而布氏田鼠的精子形态具有显著的种属特异性。结论:本实验所建立的布氏田鼠封闭群可作为一种中国特有的实验动物。

Objective：With drug cleaning technique and random mating,we established the cleaning grade closed colony of Microtus brandti.Methods：Microtus brandti have been continuously breeding for 6 generations.Results：Vagina smear and appearance of vagina observation using papanicolaou staining method demonstrated that the estrous cycle of adult female Microtus brandti is about 5 days.The appearance of sperm has significant species traits.Conclusion：The closed colony of Microtus brandti established in this study could be a China＇s peculiar laboratory animals.