摘要
目的:建立以氘代同位素为内标测定食品中呋喃的HSGC-MS方法。方法:试样中加入内标物d4-呋喃,顶空50℃恒温30 min后,用PLOT Q石英毛细管柱气相色谱分离,采用选择离子监测(SIM)的质谱扫描模式进行定量分析。结果:呋喃在5~1000 ng范围内线性良好,方法检测限(S/N≥3)为2.0 ng/g;不同基底样品中高低添加浓度的加标回收率为86.1%~97.4%,相对标准偏差值(RSD)均小于10%,共检测食品样品45份,检出浓度在〈2.0~84.2 ng/g之间。结论:该方法灵敏度和准确度高,适合于食品中呋喃的常规检测。
Objective:To develop a highly sensitive method for the determination of furan in foods using capillary gas chromatography with mass spectrometric detection and incorporating the use of a deuterated internal standard.Methods:After addition of internal standard d4-furan,food samples were heated at headspace incubation temperature 50℃ for 30 min.Furan was separated by gas chromatography on a PLOT Q capillary column,identified and quantified by mass spectrometry in SIM mode.Results:The correlation coefficients were good in the concentration range of 5~1000 ng.The limits of detection were 5~1000 ng/g.The recoveries were 86.1%~97.4%,and the relative standard deviation(RSD) low than 10%.45samples were determined,the concentration was 2.0~84.2 ng/g.Conclusion:The method is sensitive and precise for routine determination of furan in foods.
出处
《中国卫生检验杂志》
CAS
2009年第12期2790-2792,共3页
Chinese Journal of Health Laboratory Technology
关键词
呋喃
顶空气相色谱-质谱法
食品
Headspace gas chromatography-mass spectrometry(HS-GC-MS)
Furan
Foods