固相萃取-高效液相色谱法测定水产品中的三聚氰胺

Determination of melamine in aquatic products by SPE-HPLC

目的：建立用固相萃取-高效液相色谱法测定水产品中三聚氰胺的高效液相色谱法。方法：水产品中的三聚氰胺经阳离子交换固相萃取柱净化后,采用Kromasil C18色谱柱（4.6 mm×250 mm,5μm）分离,以庚烷磺酸钠-柠檬酸缓冲液：乙腈（85：15）为流动相,由高效液相色谱-紫外检测器分析测定。结果：该方法在10～100μg/ml的范围内具有良好的线性关系（r=0.9993）;加标回收率为80.1%～88.9%;RSD为2.1%～5.0%;方法检出限为1.6 mg/kg。结论：该方法操作简便快捷、净化富集效果好、重现性较高,能满足实际样品的日常检测需求。

Objective：To establish a SPE-HPLC method for the determination of melamine in aquatic products.Methods：Melamine in aquatic products was cleaned-up by SPE column,then separated by Kromasil C18 column（4.6 mm×250 mm,5 μm）with ultraviolet detection.The mobile phase consisted of sodium heptanesulfonate-citric acid buffer solution：acetonitrile（85：15）.Results：The linear ranges were 10~100 μg/ml（r=0.9993）,the recoveries were in the range of 80.1%～88.9%,the relative standard deviations were 2.1%～6.1%,and the determination limit was 1.6 mg/kg.Conclusion：This method is of good reproducibility and simple operation and it could be applied to the determination of the aquatic products.