类风湿性关节炎患者IL-15、TNF-α的水平变化及意义

Change of IL-15,TNF-α levels in patients with rheumatoid arthritis and its clinical significance

目的:研究类风湿性关节炎(RA)患者血清IL-15、TNF-α的变化及其临床意义。方法:收集34例RA患者,以20例骨关节炎(OA)患者和30例健康体检者作对照。采用双抗体夹心酶联免疫吸附法测定血清IL-15、TNF-α的水平,并测定类风湿因子和C反应蛋白。结果:RA患者的血清IL-15、TNF-α的含量明显高于OA组及健康体检组(P<0.05),RA患者的类风湿因子明显高于对照组(P<0.05),C反应蛋白的含量在各组间差异无统计学意义;RA组中,血清IL-15、TNF-α水平明显相关(r=0.771)。结论:IL-15、TNF-α在RA患者的疾病发展过程中发挥着重要作用,阻断其生物活性,为靶向治疗RA患者提供了新思路。

Objective：To investigate the change of interleukin-15 and tumor necrosis factor-α levels in patients with rheumatoid arthritis（RA） and their clinical significance.Methods：34 patients with rheumatoid arthritis（RA group）,20 patients with osteoarthritis（OA） and 30 healthy subjects（served as two control groups） were enrolled in the investigation.IL-15 and TNF-α levels were detected by double antibodies sandwich enzyme-linked immunosorbent assay（ELISA）.The levels of rheumatoid factor and C-reactive protein were detected as well.Results：The level of IL-15,TNF-α and rheumatoid factor in RA patients were significantly higher than those in two control groups.In contract,the level of C-reactive protein in three groups showed no significant difference.The level of serum IL-15 significantly correlated with that of TNF-α in patients with RA（r=0.771）.Conclusion：IL-15,TNF-α play an important role in the development of rheumatoid arthritis.Blocking the effects may give a new hope in treatment of RA.