应用R带显带技术对151例急性非淋巴细胞白血病行细胞遗传学分析

Cytogenetic analysis on 151 cases of acute nonlymphocytic leukemia by R-banding technique

目的:分析急性非淋巴细胞白血病(acute nonlymphocytic leukemia,ANLL)的核型状况。方法:利用短期培养法和直接法制备骨髓细胞染色体,用R带显带技术对151例初治的ANLL患者行染色体核型检查。结果:该组患者中克隆性染色体异常的有84例(56%)。异常核型主要为特异性染色体重排,共有65例,占核型异常的77%。t(15;17)(35例)和t(8;21)(23例)是最常见的结构异常。三体-8是最常见的数目异常。100%的t(7;11)见于M2,100%的t(15;17)见于M3,83%的t(8;21)见于M2,100%的t/del(11q23)见于M5。结论:应用R带显带技术,56%的ANLL患者可检出克隆性染色体异常特别是特异性染色体重排。它们和FAB分型相关,因此核型是ANLL诊断、分型、判断预后的一项重要指标。

Objective：To analyze the karyotypic status of acute nonlymphocytic leukemia（ANLL）.Methods：A total of 151cases of de novo ANLL were studied.Chromosome preparations were made on bone marrow cells using short-term culture and direct method.Karyotypes were analyzed by R-baning.Results：Eighty-four cases（56%） had clonal chromosomal abnormalities among them.The specific chromosomal rearrangements seen in 65 cases were found the major karyotypic abnormalities,accounting for 77% of the total number of karyotypic abnormalities.t（15;17）（35 cases）and t（8;21）（23 cases） were the most frequent structural abnormalities.Trisomy 8 was the most common numerical abnormality.The 100% of t（7;11） were detected in M2,100% of t（15;17） were detected in M3,83% of t（8;21） were detected in M2,100% of t/del（11q23） were detected in M5.Conclusion：By R-banding technique,56% of patients with ANLL may be found to have clonal chromosomal abnormalities which are predominantly specific chromosomal rearrangements correlated with FAB phenotype.Thus,karyotype is an important indicator for diagnosis,classification and judging prognosis of ANLL.