安颤灵对蛋白酶体抑制剂lactacystin诱导PC12细胞凋亡的影响

Effects of Anchanling on Apoptosis Induced by Proteasome Inhibitor Lactacystin in PC12 Cells

观察安颤灵含药血清对lactacystin诱导PC 12细胞凋亡的影响。方法:按照血清药理学实验方法制备安颤灵含药血清,采用lactacystin作用于PC 12细胞,建立泛素-蛋白酶体系统损伤的细胞模型。将含药血清分为大、中、小剂量组,MTT方法检测各组的细胞活力;将对数生长期的PC 12细胞分为正常组、lactacystin组(损伤组)、中药组,通过Tunel及免疫细胞化学方法,观察各组细胞凋亡情况。结果:与大、小剂量组相比,中剂量组细胞的存活率显著升高(P<0.01);与正常组相比,lactacystin组细胞凋亡指数明显增加(P<0.01);与lactacystin组相比,中药组细胞凋亡指数明显降低(P<0.01)。结论:安颤灵含药血清具有抗lactacystin诱导的细胞凋亡作用。

Objective To explore the effects of Anchanling on lactacystin inducing apoptosis in PC 12 cells. Methods Serum pharmacological method was used to prepare serum containing Anchanling. Lactacystin was used as proteasome inhibitor to create a cellular model of ubiquitin-proteasome system dysfunction in PC 12 cells. PC 12 cells were divided into three groups：lactacystin treated group, Anchanling treated group and control group. We used MTT assay to evaluate cellular viability. TUNEL staining was employed to observe the apoptosis of nervec cells. Results The AnChanLing middle dose group showed the highest cellular viability of the three dose group（P 〈 0.01）. Compared to the control group, the proportion of apoptosis in lactacystin group was increased significantly（P 〈 0.01）. The proportion of apoptosis in Chinese medicine trated group were significantly lower than thet of lactacystin treated group（P 〈 0.01）. Conclusion AnchanLing could obviously lower the neurotoxicity induced by lactacystin in PC 12 cells, which maybe relate to inhibiting the apoptosis of PC 12 cells.