厌氧菌基因诊断标本的前处理研究

The study on pre-processing of gene diagnosis specimens about anaerobic bacteria

目的探索疑似感染厌氧菌基因诊断标本采集、运送、保存的方法,提高厌氧菌标本前处理的有效性。方法对临床标本、土壤样本进行疱肉增菌、厌氧血平板、硫乙醇酸钠培养,分纯、鉴定出厌氧菌产气荚膜梭菌和破伤风梭菌,对此两种菌株用PCR方法进行体外基因扩增、测序、比对验证,用于基因芯片诊断研究。结果两种厌氧菌的成功培养鉴定离不开样本前处理的有效方法,为基因芯片诊断研究的标本前处理提供了参考。结论针对厌氧菌培养鉴定的难度,尽可能减少厌氧菌感染性疾病的漏诊、误诊,提高临床诊断、救治速度,基因芯片诊断将具有重要的临床意义。

Objective To explore the method of collection, transportation and preservation of gene diagnostic specimens for suspected anaerobic infection, and improve the availability of pre processing of specimen about anaerobic bacteria. Methods After anaerobic bacteria cultivation of different clinical specimens and soil samples with bacteria blister meat, anaerobic basic blood and sodium sulfur ethanol training flat, we identified anaerobic bacteria Clostridium perfringens and Clostridium tetanus, in vitro the two strains were used to gene amplification, sequencing and verification for the gene chip diagnosis by PCR method. Results Effective pre-processing of specimen about anaerobic bacteria was prerequisite of successful culture and identification. The study provided reference for pre-processing of gene diagnosis specimens. Conclusion It is of important clinical significance for performing gene chip thcbnique to reduce missed diagnosis and misdiagnosis of anaerobic infectious disease more as possible.