黄芪微乳载入修饰后胶原对人脐静脉内皮细胞增殖的影响

Effects of Astragalus mongholicus microemulsion modified-collagen on proliferation of human umbilical vascular endothelial cells in vitro

目的:研究黄芪微乳载入修饰后胶原对人脐静脉内皮细胞(HUVEC)增殖的影响,进一步探讨黄芪微乳对HUVEC增生的作用及其机制。方法:体外培养HUVEC细胞,用含1%FCS的RPMI-1640培养液同步24h后,分为2组进行观察:空白微乳胶原组和黄芪微乳胶原组,并设定空白微乳和黄芪微乳的浓度分别为16.90、8.50、4.20、2.10、1.05μg/ml等5个浓度,每组每个浓度设3个复孔。干预48h后,采用MTT法和流式细胞术检测各组细胞的增殖情况;实时荧光定量RT-PCR检测细胞内血管内皮细胞生长因子(VEGF)mRNA表达。结果:干预48h后,在浓度分别为16.9、8.5、4.2μg/ml的黄芪微乳胶原组HUVEC的吸光度值、细胞分裂增殖指数和VEGFmRNA表达均明显高于相应浓度的空白微乳胶原组(P<0.05,P<0.01)。结论:黄芪微乳能促进HUVEC的增殖,并上调HUVEC的VEGFmRNA表达,推测黄芪微乳可能具有促进血管生成的作用。

Objective：To investigate the effect of Astragalus mongholicus microemulsion modified-collagen on proliferation of human umbilical vascular endothelial cells （HUVEC） in vitro. Methods：Cell line of HUVEC was cultured and all experiments were performed using the cells at the third passage. After synchronization of cells growth,HUVEC was divided into blank microemulsion modified- collagen group and Astragalus mongholicus microemulsion modified-collagen group. Each group was exposed to the concentrations of 16.90,8.50,4.20,2.10 and 1.05 μg / ml,respectively. The proliferation of HUVEC was measured by MTT and flow cytometry assay. The real-time quantitative PCR was used to detect the expression of vascular endothelial cell growth factor （VEGF） mRNA in HUVEC. Results：After co-culturing for 48 h,the proliferation and VEGF mRNA expression of HUVEC in Astragalus mongholicus microemulsion modified-collagen group at the concentrations of 16.9,8.5 and 4.2 μg / ml significantly increased compared with the groups on the same concentrations in blank microemulsion modified-collagen control （P 〈 0.05,P 〈 0.01）. Conclusion：Astragalus mongholicus microemulsion not only promotes the proliferation of HUVEC,but also upregulates the expression of VEGF mRNA. This study demon- strateds that Astragalus mongholicus microemulsion may promote angei-anagenesis.