依达拉奉对在体成年兔肺缺血再灌注损伤的保护作用

Protective effect of Edaravone on adult rabbit pulmonary injury induced by ischemia-reperfusion in vivo

目的:观察氧自由基清除剂依达拉奉对在体成年兔肺缺血再灌注损伤的影响,从而探讨其可能的保护作用机制。方法:由南京农业大学实验动物中心提供的27只健康成年新西兰白兔,随机分为3组:假手术组(Sham,n=6),缺血再灌注组(IR,n=10)和依达拉奉干预组(IR+Edaravone,n=11)。用小儿肠钳钳夹左肺门60min,然后再开放60min制备成年兔在体肺缺血再灌注模型。依达拉奉干预组于阻断前和开放前各予静脉注射依达拉奉3mg/kg。对比观察各组肺组织中超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-PX)活力、过氧化氢酶(CAT)活力、总抗氧化能力(T-AOC)、抑制羟自由基能力(IHR)、髓过氧化物酶(MPO)含量,肺组织湿干重比(W/D),并在光镜下比较组织形态学改变。结果:依达拉奉干预组与IR组相比,SOD、GSH-PX、CAT与T-AOC活力显著增高,MDA、MPO含量及W/D显著降低(P<0.05);IR组镜下见有肺间质增宽水肿,大量炎症细胞浸润,肺泡腔中可见明显出血、渗出,部分肺泡萎陷不张;而依达拉奉干预组肺组织上述改变明显减轻。结论:在体成年兔肺缺血前后用依达拉奉处理,可减轻缺血再灌注损伤,其机制可能与依达拉奉自由基清除和抗氧化作用有关。

Objective：To investigate the protective effect of free radical scavenger edaravone on adult rabbit lung ischemia-reperfusion injury in situ and the possible mechanisms. Methods：Twenty-Seven healthy New Zealand White adult rabbits were subjected to sham operation（n=6）,IR（n=10）,or edaravone plus IR（n=11）. Ischemia-reperfusion was induced by clamping the left pulmonary hilum for 60 minutes and then removal of the clamp for another 60 minutes. Edaravone （3 mg/kg,intravenous） was given before and after ischemia. The activity of superoxide dismutase （SOD）,glutathione peroxidase （GSH-PX）,catalase （CAT）,total anti-oxidation capability （T-AOC） and inhibiting hydroxy radical capability （IHR） in lung tissues,content of malondialdehyde （MDA） and myleoperoxidase （MPO）,wet to dry ratio of lung tissue weight （W / D） were measured respectively,and lung tissues were prepared for light microscopic evaluation. Results：Compared with IR group,edaravone treatment markedly enhanced the activity of SOD,GSH-PX,CAT and T-AOC, while the MDA,MPO,W / D values were significantly reduced（P 〈 0.05）. Pathologic evaluation showed alveoli injury,hemorrhage,white blood cell infiltration and interstitial edema in group IR were obvious,while which were lessen markedly in group with edaravone treatment. Conclusion：Treatment with edaravone can attenuate ischemia-reperfusion injury in adult rabbit lung in vivo. The protective mechanism might be mediated by its free radical scavenging and antioxidant action.