摘要
对羊栖菜(Hizikia fusiformis(Harv)Okamura)养殖中常见的3个品系进行了DNA指纹分析及遗传变异的研究,构建了其遗传指纹图谱,分析了不同种群的遗传关系,为羊栖菜的种质鉴定及选育提供了理论依据。运用RAPD分子标记技术,对5个羊栖菜的种群中共125个个体进行了分析,从300个引物中筛选出12条随机扩增引物共扩增135个位点,多态位点比率为84.4%。从中选择了4个多态性位点,构建了DNA指纹图谱。相关结果对羊栖菜遗传选育和种质鉴定等有参考价值。
In this study, we analyzed the genetic distances and constructed the fingerprints of the three good strains, which have been widely applied in the production. It was indicated that： RAPD （random amplified polymorphic DNA） was applied to analyzing 125 individuals from the 5 populations of Hizikia. A total of 135 amplification loci were obtained from 12 selected primers after screening and P% （the percentage of polymorphic loci） is 84.4%. The yielded 4 stable and clear bands were selected to construct a fingerprint for discrimination of the 5 populations. Our results provide the tools for the Hizikia＇s genetic breeding and germplasm identification in the future.
出处
《海洋科学》
CAS
CSCD
北大核心
2009年第12期30-33,共4页
Marine Sciences
基金
国家自然基金项目(40618001)
洞头县科技计划项目(N2006Y11B)
