桐花树多酚氧化酶同源基因片段的克隆及序列分析

Cloning and sequence analysis of polyphenol oxidase homologous gene from Aegiceras corniculatum(L.) Blanco

以桐花树(Aegiceras corniculatum(L.)Blanco)叶为材料,采用同源PCR克隆策略,扩增桐花树多酚氧化酶(Polyphenol Oxidase,PPO)铜结合区之间的cDNA序列。该序列长度597 bp(Genbank accessionNo.GQ404509),编码了一段由199个氨基酸残基组成的多酚氧化酶保守区片段。桐花树PPO保守区片段具有PPO铜结合区A(CuA)的特征信号序列Hnswl.FFp FHRyyLyff E。同源比对表明,该序列与GenBank上其他已有的植物多酚氧化酶氨基酸序列的同源性都在65%以上。

A PCR-based homologous cloning strategy was used to identify Polyphenol Oxidase （PPO） genes from the leavess of Cornicnlate Aegiceras （Aegiceras corniculatum （L.） Blanco ）. The results of sequence analysis indicate that A 597 bp eDNA with a Cu-binding region encoding a 199 amino acids actin ortholog was successfully cloned and characterized （GenBank accession No. GQ 404511）. The PPO contains the CuA-binding region signature sequence of PPO family （Hnswl. FFpFHRyyLyffE）. Multiple alignment analysis of amino acid sequences of the putative PPO conserved regions in A. corniculatum was more than 65% homologous with the best scoring results from similarity searches of sequence databanks.