清肺口服液对3I、7b型腺病毒感染人胚肺成纤维细胞TNF-α mRNA基因表达的影响

Effect of Qingfei Oral Liquid on Tumor Necrosis Factor-α mRNA gene expression of human embryonic lung fibroblasts induced by adenovirus Type3I,7b

目的:探讨清肺口服液含药血清对腺病毒3I、7b感染的人胚肺成纤维细胞之肿瘤坏死因子-α(TNF-α)mRNA基因表达的影响。方法:以3I、7b型腺病毒分别攻击体外培养的人胚肺成纤维细胞,制备清肺口服液含药血清,作用于该细胞,另设正常细胞组,病毒对照组,利巴韦林组,采用原位杂交法检测不同组细胞TNF-α的mRNA基因表达,并进行比较。结果:病毒对照组较正常细胞组之TNF-α mRNA基因表达明显增强(P<0.01),含药血清组可显著降低3I、7b型腺病毒攻击后细胞TNF-α的mRNA表达(P<0.01)。结论:①腺病毒感染可使人胚肺成纤维细胞TNF-α mRNA表达增高;②清肺口服液可下调TNF-α的mRNA表达,这可能是其抗病毒作用的机制之一。

Objective：To study the effect ofQingfei Oral Liquid medicated serum on the tumor necrosis factor-α（TNF-α） mRNA gene expression ofhuman embryonic lung fibroblasts induced by adenovirus Type3I, 7b.Methods：We determined the TNF-α mRNA ofADV-infected human embryonic lung fibroblasts before and after adding the medicated serum by in situ hybridization.Results：Adenovirus could up-regulate the TNF-αmRNA ofhuman embryonic lung fibroblasts（P〈0.01）.While Qingfei Oral Liquid could down-regulate them（P〈0.01）.Conclusion：Infection ofadenovirus can induce the increase ofTNF-α mRNA expression in human embryonic lung fibroblasts.Qingfei Oral Liquid played an antiviral role by regulating some cytokine genes.