应用磁共振显像动态观察脑疏宁对大鼠创伤脑水肿的干预作用

Dynamic observation on interventional effect of Naoshuning on traumatic brain edema in rats by using MRI

目的通过观察大鼠脑挫裂伤后脑水肿的磁共振显像(MRI)动态变化,分析中药脑疏宁对脑水肿的干预效果。方法SD雄性大鼠114只,随机分为脑疏宁组、模型组和假手术组。采用自由落体撞击法建立大鼠脑挫裂伤模型,术后连续动态观察T_2加权像(T_2WI)病灶区信号强度(SI)与体积变化,采用干湿重法测定脑组织含水量。结果外伤后术侧大脑半球见T_2WI高信号改变,1 d时信号强度最高、体积最大,至7 d下降至最低点,与脑组织含水量的变化一致。脑疏宁组各时间点T_2WI信号强度、水肿体积及脑含水量均显著小于模型组(P<0.05),术后7 d水肿吸收率显著高于模型组(P<0.005)。结论无创性MRI检查能够对大鼠脑外伤及用药后脑水肿程度作出定量分析,是评价药物疗效的可靠手段;中药脑疏宁能够促进大鼠脑挫裂伤后脑水肿的吸收。

Objective To analyse the effect of Naoshuning on brain edema through observing the dynamic changes of magnetic resonance imaging（MRI） in rats with brain contusion and laceration.Methods All 114 male SD rats were randomly divided into Naoshuning group,model group and sham-operation group. The rat model of brain contusion and laceration was established by free-falling impact according to Feeney＇s method.After the operation the dynamic changes of MRI signal intensity（SI） and volume in T2 WI focus were observed consecutively,and the brain water content was determined by using dry-wet method.Results There were changes observed in increased SI of T2 in the cerebral hemisphere with operation.SI got the highest level and volume on the first day and then decreased gradually and reached the lowest level on the seventh day,which showed a parallel course with brain water content.In Naoshuning group SI in T2 WI focus,edema volume and brain water content were significantly lower than those in the model group at all time points（P〈0.05）.The absorption rate of edema in Naoshuning group was significantly higher than that in the model group（P〈0.005） seven days after the operation. Conclusion The non-invasive MRI examination can be used to analyse quantitatively the degree of brain trauma and edema in rats after the treatment with Naoshuning,which is reliable for review the curative effect.Naoshuning can improve the absorption of edema after brain contusion and laceration.