利用核磁共振代谢组学技术研究四物合剂对化疗后大鼠代谢的影响

Influence of Siwu Mixture on metabolism in rats after chemotherapy by applying NMR metabonomics technique

目的研究四物合剂对化疗药顺铂腹腔注射后大鼠体内代谢产物的影响。方法将6只大鼠随机分为实验组、模型组和正常组,每组2只。实验组及模型组腹腔注射顺铂后,前者灌胃四物合剂,后者灌胃纯净水;正常组腹腔注射生理盐水后,灌胃纯净水。收集灌胃后各组大鼠每日尿液,测定1 H-NMR谱。结果经多元统计分析,实验组、模型组及正常组的代谢产物有明显差异,其中乙酸、丙氨酸、乳酸、马尿酸等化合物含量差异性较大。结论提示四物合剂对肝肾功能具有一定的保护作用。

Objective To study the influence of Siwu Mixture on metabolites in rats after the intraperitoneal injection of chemotherapeutics-Cisplatin.Method The rats（n=6） were randomly divided into the experimental group,model group and normal group（each n=2）.The experimental group and model group were respectively given Siwu Mixture and purified water intragastrically after the intraperitoneal injection of Cisplatin,and the normal group was given purified water intragastrically after the injection of normal saline solution.The urine were collected from the rats in all groups every day and the spectroscopy of 1H-nuclear magnetic resonance（1H-NMR） was determined.Result After multivariate statistical analysis the metabolites of the experimental group,model group and normal group were different significantly,especially acetic acid,alanine,lactic acid and hippuric acid.Conclusion The result of the study indicates that Siwu Mixture can protect the functions of the liver and kidney.