灵芝漆酶催化直接耐晒翠蓝GL脱色条件的优化

Optimization of Conditions in Decolorization of Direct Fast Turquoise Blue GL Catalyzed by Laccase from Ganoderma lucidum

采用灵芝菌株发酵所得的漆酶,对酞菁类染料直接耐晒翠蓝GL进行了催化脱色实验,确定了脱色反应的最适条件。结果表明:单独使用灵芝漆酶粗酶液对直接耐晒翠蓝GL具有很好的脱色效果。其最适脱色pH为3.0,最适脱色温度为40°C,最适漆酶用量是40U/mL,最适染料浓度为60mg/L。以上述最适脱色条件对直接耐晒翠蓝GL进行脱色实验,反应70min,脱色率可达94.3%。研究结果显示,所试灵芝漆酶在印染废水治理方面具有良好的应用前景。

The decolorization experiments of direct fast turquoise blue GL were conducted with the crude laccase from Ganoderma lucidum in submerged fermentation. The optimum decolorization conditions were determined. The results showed that the crude laccase from Ganoderma lucidum had distinct decolorization efficiency on direct fast turquoise blue GL. The optimum pH was 3.0, The optimum temperature was 40℃, The optimum enzyme dosage was 40 U/mL, the optimum dye concentrations was 60 mg/L. Under the best conditions optimized above, the decolorization rate of direct fast turquoise blue GL was 94.3% after 70 min catalyzation with the laccase from G lucidum. Our results suggested that the laccase from Ganoderma lucidum could be very useful in the textile-dye decolorization and waste-water purification.