帕金森病模型大鼠纹状体mGluR5的表达变化及意义

The altered expression of metabrotropic glutamate receptor subtype 5 (mGluR5) in the striatum of rat wtih Parkinson's disease

目的:探讨PD模型大鼠纹状体mGluR5的表达变化及意义。方法:将SD大鼠随机分为正常对照组,PD模型组和亚型选择型mGluR5拮抗剂SIB-1893+PD组,通过免疫组织化学方法观察多克隆抗体mGluR5在大鼠纹状体的表达变化。结果:正常对照组中大鼠纹状体有丰富的mGluR5表达;PD模型组中大鼠纹状体mGluR5表达明显下降;在亚型选择性mGluR5拮抗剂SIB-1893+PD组中,大鼠纹状体mGluR5表达又上调。结论:PD模型大鼠纹状体mGluR5表达下降可能是中枢神经系统在DA能神经缺失以后引起的一种病理性代偿反应,mGluR5可能参与了PD的发生、发展过程。亚型选择性mGluR5拮抗剂有助于减低间接通路的传导,对DA神经元有保护作用,可望成为治疗PD的一种副作用小的新药。

Objective： To explore the expression of metabotropic glutamate reeeptoe subtype 5 （mGluR5） in the striatum of normal and Parkinson＇s disease model rat and the potential mechanism of the functional disturbance of striatum recognition . Methods ：All rats were divided randomly into three groups： normal group, PD group , and SIB-1893 （ antagonist of mGluR5） ＋ PD group. Polyclonal antibody specific of mGluR5 was used to observe the immunoreaetion of mGluR5 in the striatum. Immunobistoehemistry combined with the image quantitative analysis was used. Results ：The expression of mGluR5 in the rats of A group was high in the striatum. Whereas an initial down regulation of mGluRS was observed in all the regions of the striatum of rats from B group. At the same time the expression of mGluR5 in the group of C. was increased compared to that in group B. The expression of mGluR5 is mainly on membrane. Conclusions： The expression of mGluR5 in the rats of PD model in striatum was descend. Probably a kind of endogenous protection system maybe take place in the central nervous system after the absence of DA-contained neurone. The expression of mGluR5 is up-regulated through the use of antagonist of mGluR5 SIB 1893 indicated the protective effect on neurone. It can reduce the indirect pathway and will be a new method for clinical treatment.