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脑通灵对大鼠脑缺血再灌注神经元形态和Caspase-9mRNA表达变化的影响 被引量:1

The effects of naotong ling on Neuron morphology and expression of caspase-9 mRNA in rats with cerebral ischemia reperfusion
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摘要 目的:探讨脑通灵对大鼠脑缺血再灌注损伤的保护作用。方法:应用颈动脉血管引流法建立大鼠脑缺血再灌注动物模型,采用Caspase-9mRNA原位杂交技术,DAB显色,光镜观察细胞浆呈棕黄色为阳性细胞。经图像分析阳性细胞的多少,观察脑通灵对大鼠脑缺血再灌注损伤的保护。结果:脑通灵治疗组与模型对照组比较,阳性细胞平均数密度减小,平均灰度值增大,组间比较有明显差异(P<0.05)。结论:观察脑通灵对大鼠脑缺血再灌注损伤有一定的保护作用。 Objective.. To observe the protective effect of nao tong ling on ischemia reperfusion injury in rat brain. Methods :We used the modified Bannister method to establish the modle of focal cerebral ischemia reperfusion injury in rats to study the effect of nao tong ling on Caspase-gmRNA in cerebral cortex in the modle rats. Results:nao tong ling decreased average numerial and increased average gray value ,there was obvious difference between the groups(P〈0.05). Conclution :nao tong ling has a protective effect on rat brain damage induced by focal cerebral ischemia reperfusion.
作者 陈晓春
出处 《陕西医学杂志》 CAS 2009年第12期1587-1589,共3页 Shaanxi Medical Journal
基金 河北省科学技术研究与发展指导计划项目(NO:072761916)
关键词 脑缺血/并发症 再灌注损伤 神经元 @Caspuse-9mRNA动物 实验 大鼠 Brain ischemia/complications Reperfusion injury Neurons @Caspase-9mRNA Animals laboratory Rats
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