一株农药降解生防细菌的分离与鉴定

Isolation and Identification of a Pesticide-degrading and Biocontrol Bacterium

从花生根际土壤中分离到一株对多种土传植物病原真菌具有拮抗作用的生防细菌B3,该菌能够以100mg/L高效氯氰菊酯、毒死蜱和吡虫啉为唯一碳源生长,7d的降解率分别为54.42%、57.44%和49.24%,显示了该菌在植物病害生物防治和土壤生物修复方面具有潜在的开发价值。通过形态特征、生理生化及16S rRNA同源性序列分析,鉴定该菌为多粘类芽孢杆菌(Paenibacillus polymyxa)。

One biocontrol bacillus B3 was isolated from peanut rhizosphere, which showed a broad inhibition spectrum against various soil-borne plant phytopathogenic fungi. It was also found to be capable of utilizing beta-cypermethrin, chlorpyrifos and imidacloprid as the sole source of carbon for growth. The ex- tent of degradation of them at initial concentration of 100 mg/L was 54.42%, 57.44% and 49.24% respec- tively within 7 days. Strain B3 was identified as Paenibacillus polymyxa based on morphological, physio- logical-biochemical properties, and 16S rRNA sequence analysis. This study indicated that strain B3 had potential application in biological control and soil bioremediation.