正交实验优选光合细菌混合菌群产氢的最佳条件

Optimization of Hydrogen Production Conditions of Photosynthetic Bacteria Group by Orthogonal Test

对光合细菌混合菌群产氢影响因子进行了实验研究。通过单因素实验和正交实验,系统考察了碳源、氮源、碳源浓度、氮源浓度、初始pH值、光照方式、接种量等因素对产氢量的影响,实验得出最佳工艺条件为:采用3号菌群,碳源为葡萄糖,碳源浓度为3g/L,氮源为尿素,氮源浓度为9g/L,接种量为10%,pH值为8.5,光照方式为12h光照-12h黑暗交替光照,培养温度为30°C。菌种、碳源、碳源浓度、氮源是影响产氢量的重要因素。

The influence factors for hydrogen production using photosynthetic bacteria group were studied. The effects of carbon sources, nitrogen sources, carbon concentration, nitrogen concentration, initial pH value, illumination mode and inoculation amount on hydrogen production were investigated by experiment of single factor and orthogonal experiment. The optimum process conditions were as follows： the best bacte- ria group was number 3, the glucose was carbon source and concentration of carbon source was 3 g/L; the urea was nitrogen source and concentration of nitrogen source was 9 g/L; the inoculation amount was 10%; initial pH value was 8.5; the illumination mode was 12 h light and 12 h dark alternation; incubation temperature was 30℃. The main factors affecting hydrogen production were strain, carbon sources, carbon source concentration and nitrogen suorces.