深红红螺菌吸氢酶缺失突变株在管式光合反应器中的产氢

Hydrogen Production of Rhodospirillum rubrum Uptake Hydrogenase Deficient Mutant in Tubular Photobioreactor

本研究设计了一种2L分体式管式光合反应器,并研究了深红红螺菌(Rhodospirillum rubrum)吸氢酶缺失突变株在该反应器中分别利用人工光源(持续光照与光暗交替)和自然光的产氢规律。结果表明在人工光照条件下R.rubrum的产氢可维持5d,持续光照和光暗交替条件下(12h:12h)的氢产量可分别达到5752mL/PBR±158mL/PBR和5012mL/PBR±202mL/PBR;自然光条件下,最适产氢光照强度为30000Lux^40000Lux;在此光照条件下,R.rubrum产氢可维持6d^10d,最高氢产量可达到2800mL/PBR。尽管利用自然光的氢产量比利用人工光源氢产量低,但是利用自然光的产氢比较经济,并且该光合产氢系统操作简单,该工艺有望开发为低成本的光合细菌产氢技术。

A 2 L tubular photobioreactor （PBR） was designed, and hydrogen production by Rhodospirillum rubrum uptake hydrogenase deficient mutant in this PBR under artificial illumination condition （including continuous illumination and light/dark cycle condition） and natural day/night cycle was investigated, respec- tively. Hydrogen production by R. rubrum uptake hydrogenase deficient mutant under artificial illumination lasts five days, and hydrogen yield under continuous illumination and light/dark cycle （12 h： 12 h） reaches 5752 mlJPBR ± 158 mL/PBR and 5012 mL/PBR ± 202 mL/PBR, respectively. Under natural day/night cycle, hydrogen production lasts 6-10 days and the maximal hydrogen yield of R. rubrum reaches 2800 mL/PBR with the optimal light intensity of about 30000～40000 Lux. Hydrogen production under natural day/night cycle condition was lower than that under artificial illumination, however, it would be a promising technology of hydrogen production due to low cost and ease of operation of hydrogen production system.