一步法分离鸡蛋清中三种主要过敏原的研究

Simultaneous Separation of Three Major Allergens in Hen's Egg White

为快速高效分离鸡蛋中主要过敏原开展鸡蛋过敏的研究,本实验采用DEAE-Sepharose FF色谱柱一步分离鸡蛋中的三种主要过敏原卵白蛋白、卵转铁蛋白和溶菌酶,通过SDS-PAGE比对,纯度均高于相应标准品纯度,得率分别为87.66%、55.87%和92.80%。本实验操作简便、设备简单、分离效率高,是一种分离鸡蛋过敏原的好方法。

Hen＇s egg is one of the most common causes of food allergy.In order to obtain egg white allergens as a material for further studies,three major allergens,i.e.,ovalbumin,ovotransferrin and lysozyme,were separated by DEAE-Sepharose FF chromatography,with recovery rates of 87.66%,55.87 % and 92.80% and purities of higher than 90%,respectively.