高速剪切与微波辅助偶联提取鲜葛中葛根素

Combined High Speed Shear and Microwave Treatment for Extraction of Puerarin from Radix Puerariae(Kudzu Root)

以湘西野葛根为原料,经高速剪切破坏其组织细胞,偶联微波辅助提取其葛根素。实验探讨剪切时间、剪切速度、微波平均辐射功率、辐射时间对葛根素提取率的影响。并采用响应面法试验设计,确定其最佳提取工艺。实验结果表明最佳工艺参数为剪切时间6min,剪切速度6000r/min,微波辐射时间20s,微波功率467.27W,获取葛根素含量2.05mg/g。

With the aim to develop an optimal process using 40% ethanol based on sequential treatment with high speed shear followed by microwave for the extraction of puerarin from Radix Puerariae,root of kudzu Pueraria lobata（Willd.） Ohwi,which was grown in western Hunan province,this investigation was designed using single factor method,central composite and response surface methodology for exploring the effects of crucial technological parameters,including shearing time,shearing speed,microwave radiation power and radiation time on puerarin extraction.Results showed that the optimum extraction conditions were as follows：shearing time 6 min,shearing speed 6000 r/min,microwave radiation power 467.27 W,and radiation time 20 s.Under such conditions,a content of puerarin in final extract of 2.05 mg/g was obtained.