丝素蛋白乙酰化修饰的工艺研究

Acetylation Modification of Fibroin Protein

研究丝素蛋白乙酰化的工艺条件,主要对丝素蛋白乙酰化工艺参数——丝素蛋白浓度、乙酸酐用量、反应时间、反应温度、反应pH值作探索,用正交试验确定最佳的工艺参数。结果表明,乙酰化的最佳工艺条件为丝素蛋白浓度2%、乙酸酐用量15%、反应时间20min、反应温度60℃、反应pH8.5,乙酰化程度达到47.8%。乙酰化的丝素蛋白可以作为优良的表面活性剂。

Fibroin protein was modified by acetylation,and some key technique parameters such as fibroin protein concentration,acetic anhydride dosage,reaction temperature,time and pH value were studied and optimized by conducting orthogonal test.Results showed that the optimum acetylation reaction was carried out at 60 ℃,pH 8.5 for 20 min with fibroin protein concentration 2% and anhydride concentration 15%.Under such conditions,the acetylation ratio was up to 47.8%.