响应面法优化鲨鱼硫酸软骨素的提取条件

Use of Response Surface Analysis for Optimization of Chondroitin Sulfate Extraction from Shark Fin Cartilage

采用响应面分析法,研究不同提取条件(温度、时间、碱浓度)对鲨鱼硫酸软骨素(ChS)得率的影响,建立提取条件与得率之间的数学模型,并进一步研究不同的超声波处理时间对硫酸软骨素提取率和纯度的影响,确定适宜的硫酸软骨素提取条件。结果:1)采用响应面分析法建立提取条件与硫酸软骨素得率的数学模型为ChS(%)=14.56-0.34x2-0.34x1x2-0.82x12-1.45x22-0.47x32,其中碱提温度对硫酸软骨素得率影响最为显著,其次为碱液浓度;2)通过响应面交互作用分析与优化,鲨鱼硫酸软骨素适宜的碱提条件为碱液浓度4%、碱提温度35℃、碱提时间3h,ChS得率为14.50%;3)在优化的碱提条件处理之前利用超声波处理20min,硫酸软骨素得率可提高到18.07%,纯度为91.58%。

Chondroitin sulfate（ChS） was extracted from shark fin cartilage,and the effects of alkaline concentration（x1）,extraction temperature（x2） and time（x3） on the yield were studied.By conducting response surface analysis,the optimum extraction conditions were obtained,and a multiple regression model was set up between the extraction conditions and the yield of ChS,then the effect of ultrasonic time on the yield and purity of ChS was studied.The mathematical model between the extraction conditions and yield of shark ChS as follows：ChS（%）=14.56-0.34x2-0.34x1x2-0.82x12-1.45x22-0.47x32.The extraction temperature significantly affected the yield of ChS,followed by the alkaline concentration.According to the response surface analysis,the optimal extraction conditions were as follows：temperature 35 ℃,time 3 h,alkaline concentration 4% with the yield of ChS 14.50%.The yield and purity of ChS increased to 18.07% and 91.58% respectively,if shark fin cartilage was processed by ultrasound for 20 min prior to alkaline extraction.