摘要
采用气相色谱法、高效液相色谱法和薄层层析法研究猪屎豆种子胶多糖的单糖组分及其含量,实验结果表明,猪屎豆种子胶多糖主要是由半乳糖和甘露糖组成的半乳甘露聚糖,气相色谱法测定其单糖组分百分含量分别为25.377%和60.282%;半乳糖与甘露糖物质的量的比为1:2.375。液相色谱测定其单糖组分百分含量分别为29.261%和70.739%;半乳糖与甘露糖物质的量的比为1:2.417。结论:气相色谱法与HPLC测定结果非常接近。该方法简便、实用、可靠,适用于半乳甘露聚糖的单糖组分定性和定量的分析。
Monosaccharides composition of the polysaccharide gum from Crotalaria mucronat seed were analyzed using thin layer chromatography（TLC）,GC and HPLC methods.GC analysis indicated that there were 25.377% of D-galactose and 60.282% of D-mannose with their molar ratio at 1：2.375,while HPLC analysis indicated that there were 29.261% of D-galactose and 70.739% of D-mannose with the molar ratio at 1：2.417.The D-galactose/D-mannose molar ratio results obtained from GC and HPLC analysis were close.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第22期264-267,共4页
Food Science
基金
广东省自然科学基金项目(06028879)
韩山师范学院2008年教授基金项目(510076)
