摘要
目的:建立果汁类饮料中抗坏血酸(VC)含量的HPLC检测方法。方法:采用LunaC18(2)色谱柱(150mm×4.6mm,5μm);流动相:0.1%草酸溶液,流速:1ml/min;检测波长:245nm,柱温:30℃。结果:峰面积(Y)与浓度(X)在3.952～79.04μg/ml范围内线性关系良好(r=0.9999),3倍和10倍信噪比最小检出限为0.0007μg/ml和0.0022μg/ml,平均加标回收率(n=5)为108.3%。实验精密度(RSD)为0.54%,重复性实验RSD为0.28%,样品8h内检测结果稳定,日间测定RSD为5.59%。结论:该方法具有准确、简便、快速的特点,可用于果汁中VC含量的快速检测。
Objective：To develop a convenient and quick measurement method for ascorbic acid in juice.Methods：A reverse phase high performance liquid chromatography（RP-HPLC） method was established to determine the concentration of ascorbic acid in juice.The RP-HPLC was composed of SPD-10AVP as the UV-detector,Luna C18（2） column（150 mm × 4.6 mm,5 μm） as the solid phase,0.1% oxalic acid solution as the mobile phase with flow rate of 1.0 ml/min,UV detection wavelength at 245 nm and 30 ℃ of column oven temperature.Results：The good linear was in the range of 3.952-79.04 μg/ml（r = 0.9999） with the detection limit of 0.0007 and 0.0022 μg/ml according to 3× and 10× noise signal,respectively.The average recovery rate was 108.3%（n = 5）.The RSD was 0.54 and 0.28% in precision and reproducibility tests,respectively.The determination was stable in 8 h（RSD = 5.59%）.Conclusion：This RP-HPLC method is a suitable measurement method for ascorbic acid in juice due to its accuracy,convenience and quickness.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第22期268-271,共4页
Food Science
基金
四川省教育厅重点项目(2005A018)
四川农业大学青年创新基金项目(5304)
