间接原子吸收光谱法测定食品中的草酸

Oxalate Determination in Food Using Indirect Atomic Absorption Spectrometry

建立食品中草酸含量测定的新方法—间接原子吸收光谱。样品经处理后,加入过量且定量的氯化钙,使其与草酸根离子生成草酸钙沉淀,经离心分离后再利用原子吸收光谱(AAS)法定量测定上清液中剩余Ca2+的含量,间接计算出食品中草酸的含量。在最佳条件下,该法的线性范围为0～8.0mg/L,回收率为98.1%～98.4%。本方法精密度高、灵敏度高、检出限低,适用于食品中草酸含量的测定。

A novel method,indirect atomic absorption spectrometry,was established to determine oxalate in food.During the process of sample treatment,excessive calcium chloride was quantitatively added to generate calcium oxalate precipitation with oxalic acid.Followed by centrifugation,remaining calcium ions in supernatant were determined by atomic absorption spectrom-etry（AAS）.The content of oxalate in food was calculated indirectly.A good linear of this method was observed in the range of 0-8.0 mg/L and the recovery rate was 98.1%-98.4% under the optimal determination condition.Therefore,this novel method is suitable for the determination of oxalate in food due to its high precision,high sensitivity and low detection limit.