高效液相色谱法测胆固醇含量鉴别地沟油

Identification of Hogwash Oil-adulterated Rapeseed Oil through HPLC Detection of Cholesterol Content

利用高效液相色谱法测定地沟油中胆固醇含量,继而定性分析食用油中是否掺地沟油。实验结果表明,当待检菜籽油样品中胆固醇的含量大于0.05mg/g时,可以很明显地观察到胆固醇色谱峰,且菜籽油中胆固醇含量随地沟油的增加呈线性增加,而合格的食用植物油未检测出胆固醇。可利用此特征作为判断植物油中是否掺混了地沟油的依据。

Cholesterol content was determined with HPLC method for identification of hogwash oil-adulterated rapeseed oil.A good linear correlation between the adulteration amount of hogwash oil and the cholesterol content,and cholesterol was not detected in qualified rapeseed oil.Thus cholesterol content could be used as an indicator for identification of oil adulterated with hogwash oil with the lowest detection limit of 0.05 mg/g.