摘要
建立了虾青素手性异构体分离和检测的HPLC方法。条件:采用手性固定相色谱柱;PirkleL-亮胺酸(4.6mm×25cm,5μm);流动相:正己烷-四氢呋喃-异丙醇-三乙胺(77:17:3:3,V/V);紫外检测波长:474nm;流速:0.75ml/min。本法可应用于快速鉴别养殖三文鱼是否使用了人工合成虾青素,并可以初步判断是否为野生三文鱼。
A high performance liquid chromatography（HPLC） method was developed to identify salmon aquacultured with synthetic astaxanthin through determining relative ratio of chiral isomers of astaxanthin in salmon.Astaxanthin was extracted from flesh of salmon,passed through a Sep-Pak silica gel column for purification,and then analyzed by HPLC.The chromato-graphic conditions were Pirkle covalent L-leucine column（4.6 mm×25 cm,5μm） as the stationary phase,hexane-tetrahydro-furan-2-propanol-triethylamine（77：17：3：3,V/V） as mobile phase with flow rate of 0.75 ml/min,and detection wavelength at 474 nm.Distribution of chiral isomers of astaxanthin in wild salmon and salmon fed with natural feedstuff was similar.However,it revealed a significant difference in salmon fed with synthetic astaxanthin.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第22期318-320,共3页
Food Science
