摘要
实验采用DEAE-纤维素柱层析法分离食用菌提取物,得到3种多糖,通过对在培养乳酸菌的MRS培养基中添加1%3种多糖作为增殖因子,并作空白对照,37℃恒温培养,测定乳酸菌的生长曲线、菌液OD值与pH值,比较3种多糖对乳酸菌的促生长作用的差异得出,多糖Ⅰ、多糖Ⅱ对乳酸菌的增殖作用比较明显,多糖Ⅲ的增殖作用因大分子多糖与蛋白-多糖复合物含量较大而次之,但是3种多糖培养乳酸菌对数期期间活菌数都达到了1010cfu/mL以上,培养终止时间为12h。
The experiment separated the extractive of domestic fungus by using DEAE -cellulose column chromatography,and got three kinds of polysaccharides（PS）. It cultivated two kinds of lactic acid bacteria（LAB） with a kind of special medium which was added with 1 percent of PS as multiplication factor,cultivated at 37℃ and compared with blank sample. The experiment determined the growth curve,OD and pH of LAB to observe the differences of growth promoting effect among the three kinds of PS. There was a result that PS Ⅰ and PS Ⅱ had better growth promoting effect than PS Ⅲ for LAB. It was because that PS Ⅲ contained a great quantity of giant molecule PS and protein-PS compound. While all the viable count of LAB had exceeded 10^10 cfu/mL during the log phase when they were cultivated by the three kinds of PS, and the cultivation-ending time was 12 hours.
出处
《食品科技》
CAS
北大核心
2009年第12期261-264,共4页
Food Science and Technology
关键词
食用菌
乳酸菌
多糖
增殖因子
domestic fungus
lactic acid bacteria
polysaccharides
multiplication factor
