汉族寻常性银屑病患者IFN-γ受体2基因多态性研究

Polymorphism of interferon-γ receptor 2 gene in patients with psoriasis vulgaris in Chinese Hans

目的探讨IFN-γ受体2（IFN—yR2）基因氨基酸位点Gln64Arg多态性与中国汉族寻常性银屑病的相关性。方法采用PCR-限制性片段长度多态性（RFLP）方法及DNA测序方法，检测苏皖地区182例汉族寻常性银屑病患者和I14例正常人对照IFN—γR2基因Gln64Arg位点多态性。结果寻常性银屑病患者与正常人对照组IFN-γR2基因Gln64Arg多态性位点各基因型及等位基因频率分布差异无统计学意义（P〉0．05）。伴甲损害银屑病组与无甲损害银屑病组之间Gln64／Gln64基因型分布频率分别为57．5％和38．1％，差异有统计学意义（Χ^2=5．33，P=0．02）；Arg64等位基因（Gln64等位基因）分布频率分别为19．3％（80．7％）和30％（70％），差异也有统计学意义（Χ^2=5．03，P=0．02）。伴甲损害的银屑病患者组Gln64／Arg64基因型分布频率为29．8％，正常人对照组为49．1％，两组间分布差异有统计学意义（Χ^2=5．48，P=0．01）；Gln64／Gln64基因型分布频率在两组间分别为57．5％和35．1％，差异有统计学意义（Χ^2=6．23，P=0．01）；无甲损害组与正常人对照组之间差异则无统计学意义。在以上呼吸道感染为诱因或加重因素的银屑病组与非上呼吸道感染银屑病组之间Arg64／Arg64基因型分布频率分别为33．3％和15．5％，差异有统计学意义（Χ^2=4．94，P=0．03）；Arg64等位基因（Gin64等位基因）分布频率为51．9％（48．1％）和35，2％（64．8％），差异也有统计学意义（Χ^2=5．46，P=0．02）。结论IFN—γR2基因Gln64Arg多态性位点可能与寻常性银屑病患者是否伴有甲损害有关，也可能与以上呼吸道感染为诱因或加重因素的寻常性银屑病有关。

Objective To assess the association between the amino acid polymorphism （Arg64Gln） within the interferon-γ receptor 2 gene （IFN-γR2） and psoriasis vulgaris in Chinese Hans. Methods Blood samples were collected from 182 patients with psoriasis vulgaris and 114 healthy human controls in Jiangsu and Anhui provinces. The amino acid polymorphism （Arg64Gln） within the IFN-γR2 was examined by PCR-restriction fragment length polymorphism （RFLP） and DNA sequencing. Results No significant difference was observed in the amino acid polymorphism （Arg64Gln） within the IFN-γR2 between the psoriatic patients and healthy controls （P 〉 0.05 ）. There was a significant difference between patients with nail involvement and those without in the frequency of Gln64/Gln64 genotype （57.5% vs 38.1%, Χ^2= 5.33, P 〈 0.05）, andArg64 （Gln64） allele [19.3% （80.7%）vs30% （70%）,Χ^2=5.03, P 〈 0.05 ]. The frequencies of Gln64/Arg64 genotype and Gln64/Gln64 genotype in psoriatic patients with nail involvement significantly differed from those in the controls （29.8% vs 49.1%,Χ^2 = 5.48, P 〈 0.05; 57.5% vs 35.1%, Χ^2 = 6.23, P 〈 0.05）, while no significant difference was found between the psoriatic patients without nail involvement and controls. Moreover, significant difference was noted between patients with prior upper respiratory tract infec- tion （as inducements） and those without in the frequency of Arg64/Arg64 genotype （33.3% vs 15.5%,Χ^2 = 4.94, P〈 0.05） and Gln64 （Arg64） allele [51.9% （48.1%） vs 35.2% （64.8%）, Χ^2 = 5.46, P〈 0.05]. Conclusion The amino acid polymorphism （Arg64Gln） within the IFN-γR2 may be associated with the nail involvement and upper respiratory tract infection in patients with psoriasis vulgaris.