PU-VEGF-siRNA对裸鼠皮下舌癌移植瘤生长的影响

In vivo studies on the role of pU-VEGF-siRNA in the growth of tongue cancer xenograft

目的:探讨针对血管内皮生长因子(VEGF)的小干扰RNA(siRNA)对舌癌Tca8113细胞移植瘤在裸鼠体内生长的影响。方法:将两对特异性针对VEGF的siRNA真核表达载体(PU-VEGF-siRNA1,PU-VEGF-siRNA2),经脂质体转染人舌癌TCA8113细胞,经G418筛选后获得稳定表达,以空载体组做实验对照,以未转染舌癌细胞作空白对照。将各组细胞接种裸鼠背部皮下,每组5只,观察裸鼠肿瘤生长情况,免疫组织化学染色法观察裸鼠肿瘤组织VEGF的表达。结果:与实验对照组和空白对照组相比,实验组肿瘤生长速度明显减缓,VEGF表达降低(P<0.05)。结论:siRNA抑制VEGF的表达,可显著抑制舌癌移植瘤在裸鼠体内的生长。

Objective： To assess influence of vector-based small interfering RNA （siRNA） targeting vascular endothelial growth factor （VEGF） for human tongue squamous cell carcinoma （Tca8113） xenografts in vivo. Method： Two vector-based siRNA targeting VEGF （PU-VEGF-siRNA1, PU-VEGF-siRNA2）, and eukaryotic expression vector （experiment control） were transfected into Tca8113 ceils by lipofectamine2000, and screened with G418. Non-transfected cell was used as negative control. Twenty nude-mice were divided into 4 groups randomly, and then the transfected cells and non-transfected cell were subcutaneous injected on the back of mice, respectively. The tumor volume, weight, and growth curve of every group were compared. The protein expression of VEGF was detected by immunohistochemical technique. Result： Compared with experiment and negative controls, the growth of xeograft was significantly reduced, VEGF expression decreased in two experiment groups （P 〈 0.05）. But there were no statistically difference of those between two controls （P 〉 0.05）. Conclusion： Vector-based siRNA targeting VEGF are efficient in suppressing the growth of Tca8113 cell xenografts in vivo.